5GQF

Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-03
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.035, 143.625, 146.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.450 - 1.820
R-factor	0.18218
Rwork	0.181
R-free	0.23575
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	1.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.450	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.076	0.736
Number of reflections	116978
<I/σ(I)>	23.1	2
Completeness [%]	96.3	96.5
Redundancy	4.7	4.7
CC(1/2)		0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	0.1 M ammonium fluoride, 0.1 M MES-NaOH, 20-21% (v/v) PEG3350, 5% MPD