5GQB

Crystal structure of chitinase-h from O. furnacalis in complex with chitohepatose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 2 21 21
Unit cell lengths	49.276, 114.220, 123.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.779 - 2.700
R-factor	0.2039
Rwork	0.200
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w4r
RMSD bond length	0.010
RMSD bond angle	1.191
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	19777
<I/σ(I)>	18.67
Completeness [%]	99.9
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	0.1M HEPES pH 7.0, 30% v/v Jeffamine ED-2001