5GPO
The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.337, 47.705, 57.528 |
| Unit cell angles | 90.00, 91.63, 90.00 |
Refinement procedure
| Resolution | 28.752 - 1.701 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.25320 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.300 | |
| Number of reflections | 26314 | |
| <I/σ(I)> | 21.8 | 2.2 |
| Completeness [%] | 94.5 | 92.6 |
| Redundancy | 5.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | Ammonium sulfate, Bis-tris, PEG3350 |
