5GOY

The crystal structure of human cytosolic methionyl-tRNA synthetase in complex with methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.539, 93.133, 122.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.699 - 2.278
R-factor	0.1991
Rwork	0.196
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gl7
RMSD bond length	0.003
RMSD bond angle	0.720
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.278
Number of reflections	33132
<I/σ(I)>	24.3
Completeness [%]	95.3
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	100 mM Tris pH8.0, 200 mM magnesium chloride, 100 mM potassium sodium tartrate tetrahydrate, 16% PEG8000