5GOX

Eukaryotic Rad50 Functions as A Rod-shaped Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9766
Spacegroup name	P 1 21 1
Unit cell lengths	42.180, 61.972, 81.540
Unit cell angles	90.00, 99.75, 90.00

Refinement procedure

Resolution	28.221 - 2.405
R-factor	0.2164
Rwork	0.213
R-free	0.27090
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.302
Data scaling software	HKL-2000
Phasing software	PHENIX (dev_1745)
Refinement software	PHENIX (dev_1745)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	16270
<I/σ(I)>	42.2
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	291	28 - 30% PEG 600, 0.1 M bis-tris propane, 3% 1, 6-hexanediol, 5 mM DTT