5GOT

Crystal structure of SP-PTP, low molecular weight protein tyrosine phosphatase from Streptococcus pyogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	58.003, 58.003, 218.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.946 - 1.902
R-factor	0.2004
Rwork	0.196
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	18189
<I/σ(I)>	37.6
Completeness [%]	98.5
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	293	150 mM potassium thiocyanate, 100 mM Bis-Tris, 8% polyethylene glycol 3350, 2% D-sorbitol