5GOO
Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97916 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.772, 177.927, 181.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.790 - 2.110 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.21800 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.044 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 87561 | |
<I/σ(I)> | 25.9 | |
Completeness [%] | 97.3 | 95 |
Redundancy | 6.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5 M Lithium sulfate, 0.1 M Tris |