5GOL
Structure of acetyl-Coenzyme A synthase Alpha subunit C-terminal domain F598H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-01-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.480 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.839, 58.569, 120.729 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 31.240 - 2.110 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.060 |
| RMSD bond angle | 1.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.494 | |
| Number of reflections | 34491 | |
| <I/σ(I)> | 21.2 | 7.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MES PH 7.0 0.1M, 1.6M Ammonia Sulfate |
