5GOK

K11/K63-branched tri-Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-24
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	50.073, 52.911, 58.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.847 - 1.840
R-factor	0.2221
Rwork	0.221
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.003
RMSD bond angle	0.753
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.847	1.910
High resolution limit [Å]	1.840	1.840
Rmerge	0.124	0.490
Number of reflections	10791
<I/σ(I)>	8.35
Completeness [%]	77.0
Redundancy	3.2	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	0.2M MgSO4, 20% PEG 3350, 4mM CdCl2, PH6.0