5GOJ

Lys63-linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	40.440, 79.230, 35.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.019 - 1.549
R-factor	0.2492
Rwork	0.249
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.006
RMSD bond angle	0.999
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.019	1.610
High resolution limit [Å]	1.549	1.550
Rmerge	0.076	2.126
Number of reflections	17136
<I/σ(I)>	11.07
Completeness [%]	98.0
Redundancy	6.2	5.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M TRIS hydrochloride PH8.5, 2.0M Ammonium phosphate monobasic