5GO8
Linear tetra-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.395, 56.178, 38.721 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 23.694 - 2.210 |
| R-factor | 0.2282 |
| Rwork | 0.222 |
| R-free | 0.32220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.337 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.694 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.150 | 0.288 |
| Number of reflections | 3577 | |
| <I/σ(I)> | 7.73 | |
| Completeness [%] | 56.0 | |
| Redundancy | 3.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2M Sodium acetate trihydrate, 20% PEG 3350, PH8.0 |
