5GO7
Linear tri-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.955, 31.123, 32.180 |
| Unit cell angles | 71.54, 77.42, 89.44 |
Refinement procedure
| Resolution | 30.150 - 1.801 |
| R-factor | 0.2253 |
| Rwork | 0.219 |
| R-free | 0.30980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.150 | 1.870 |
| High resolution limit [Å] | 1.801 | 1.800 |
| Rmerge | 0.207 | 0.426 |
| Number of reflections | 6651 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 65.0 | |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2M sodium dihydrogen phosphate, 20% PEG 3350, PH 6.0 |
