5GO4

Truncated mitofusin-1, nucleotide-free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-15
Detector	RAYONIX MX-225
Wavelength(s)	0.91800
Spacegroup name	P 21 21 21
Unit cell lengths	51.799, 110.876, 112.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.467 - 2.202
R-factor	0.1771
Rwork	0.175
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	2.200
Number of reflections	62925
<I/σ(I)>	18.08
Completeness [%]	99.4
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium citrate tribasic dehydrate and 20~22% PEG3350