5GNZ
The M3 mutant structure of Bgl6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.160, 214.039, 99.327 |
| Unit cell angles | 90.00, 109.27, 90.00 |
Refinement procedure
| Resolution | 35.691 - 2.200 |
| R-factor | 0.1784 |
| Rwork | 0.177 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oin |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.894 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.098 | 0.557 |
| Number of reflections | 192920 | |
| <I/σ(I)> | 23.9 | 4.7 |
| Completeness [%] | 86.2 | 82.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25% PEG 8K, 600 mM NaCl, 100 mM Tris-HCl pH 8.0 |
