5GNW
Crystal structure of Uracil DNA glycosylase-Uracil complex from Bradyrhizobium diazoefficiens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-02-26 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.537, 89.633, 143.780 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 29.624 - 2.869 |
| R-factor | 0.2528 |
| Rwork | 0.251 |
| R-free | 0.30180 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.897 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 2.930 |
| High resolution limit [Å] | 2.869 | 2.880 |
| Number of reflections | 50661 | |
| <I/σ(I)> | 2.06 | |
| Completeness [%] | 83.2 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 290 | 20% PEG3350, 200 mM sodium citrate, 100 mM sodium citrate/citric acid |
