5GNI

The crystal structure of PECAM-1 IgL1-2 trans-homophilic dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0719
Spacegroup name	I 2 2 2
Unit cell lengths	60.522, 141.496, 169.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.447 - 3.008
R-factor	0.2234
Rwork	0.221
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	14823
<I/σ(I)>	25.12
Completeness [%]	99.7
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1M MES pH6.5, 1.8M (NH4)2SO4, 0.01M CoCl2