5GNG

Crystal Structure of BioG from Haemophilus influenzae at 1.26 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	45.950, 67.830, 67.990
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	30.392 - 1.260
R-factor	0.1483
Rwork	0.145
R-free	0.18090
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.392	1.280
High resolution limit [Å]	1.260	1.260
Rmerge	0.060	0.622
Number of reflections	105669
<I/σ(I)>	13.2
Completeness [%]	94.3	90.9
Redundancy	3.5	3.5
CC(1/2)	0.996	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.2M magnesium acetate, 0.1M sodium cacodylate, 25% PEG 8000