5GNE

Crystal structure of LapB from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 42 21 2
Unit cell lengths	152.970, 152.970, 108.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.500
R-factor	0.1826
Rwork	0.180
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amp
RMSD bond length	0.010
RMSD bond angle	1.410
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.108	0.627
Rpim	0.040	0.425
Number of reflections	42854	4641
<I/σ(I)>	18.9	3
Completeness [%]	95.1	100
Redundancy	17.4	17.8
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	285	0.1 M Sodium citrate tribasic dihydrate pH 6.0, 0.32 M Ammonium sulfate, 1.0 M Lithium sulfate monohydrate