5GND

Structure of Deg protease HhoA from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	135.219, 135.219, 113.754
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.920 - 2.500
R-factor	0.25
Rwork	0.247
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b6l
RMSD bond length	0.005
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	13981
<I/σ(I)>	59.5	2.9
Completeness [%]	100.0	100
Redundancy	11.1	10.6
CC(1/2)		0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	0.1 M Na acetate, 25%(w/v) PEG4000