5GNA

Crystal Structure of flagellin assembly related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97960
Spacegroup name	P 43 2 2
Unit cell lengths	50.446, 50.446, 184.579
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.21445
Rwork	0.214
R-free	0.22933
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a7m
RMSD bond length	0.006
RMSD bond angle	0.922
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	11357
<I/σ(I)>	87.6
Completeness [%]	100.0
Redundancy	27.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	296	5% PEG 400, 10% isopropanol, 0.2 M magnesium chloride, 0.1 M Tris-HCl pH 8.0