5GMV
LC3B-FUNDC1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.537, 86.850, 40.537 |
| Unit cell angles | 90.00, 110.86, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.250 |
| R-factor | 0.2094 |
| Rwork | 0.206 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.425 | 37.879 | 2.370 |
| High resolution limit [Å] | 2.250 | 7.120 | 2.250 |
| Rmerge | 0.032 | 0.314 | |
| Number of reflections | 12052 | ||
| <I/σ(I)> | 7.5 | 20.5 | 0.9 |
| Completeness [%] | 96.8 | 97.1 | 95.6 |
| Redundancy | 3.3 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG MME 2000, 0.1mM sodium cacodylate (pH 6.0) |
