5GMJ
Crystal Structure of GRASP55 GRASP domain in complex with JAM-B C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 132.989, 132.989, 219.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.849 - 2.986 |
| R-factor | 0.2296 |
| Rwork | 0.225 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.850 | 3.093 |
| High resolution limit [Å] | 2.986 | 2.986 |
| Rmerge | 0.179 | 0.775 |
| Number of reflections | 20363 | |
| <I/σ(I)> | 11.66 | 4.14 |
| Completeness [%] | 99.9 | 99.85 |
| Redundancy | 13.6 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | sodium formate, sodium acetate |
