5GMC
Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruiginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.442, 65.043, 93.186 |
| Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
| Resolution | 24.270 - 1.700 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cnd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.890 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.416 |
| Number of reflections | 75444 | |
| <I/σ(I)> | 12.1 | 2.5 |
| Completeness [%] | 93.4 | 93.4 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | Bis-Tris pH 6.5, Ammonium chloride, PEG 3350 |
