5GM0
Tl-gal with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-24 |
| Detector | MAC Science DIP-320 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.621, 84.392, 78.406 |
| Unit cell angles | 90.00, 109.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| Rwork | 0.242 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hl0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.073 | 0.330 |
| Number of reflections | 81697 | |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | polyethylene glycol 4000, 0.1M Na HEPES pH 7.5, 0.1M ammonium sulfate |
