5GLX
Crystal structure of a glycoside hydrolase from Thielavia terrestris NRRL 8126
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.283, 58.868, 38.380 |
| Unit cell angles | 90.00, 115.01, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.420 |
| R-factor | 0.1346 |
| Rwork | 0.133 |
| R-free | 0.16080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oa7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 3.062 | 1.420 |
| Rmerge | 0.068 | 0.055 | 0.181 |
| Number of reflections | 27662 | ||
| <I/σ(I)> | 22.1 | ||
| Completeness [%] | 99.4 | 95.9 | 99.1 |
| Redundancy | 5.2 | 5 | 4.9 |
| CC(1/2) | 0.996 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | MgCl2, Tris-Cl, PEG4000 |
