5GLI

Human endothelin receptor type-B in the ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-22
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	73.980, 147.540, 107.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.523 - 2.500
R-factor	0.25
Rwork	0.247
R-free	0.27610
RMSD bond length	0.002
RMSD bond angle	0.436
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.238	1.933
Number of reflections	20780
<I/σ(I)>	8.3	1.1
Completeness [%]	99.9	100
Redundancy	8.9	8
CC(1/2)	0.955	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.3	293	MES, PEG 350 MME, (NH4)2SO4, 1,4-butanediol