5GLH

Human endothelin receptor type-B in complex with ET-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-11
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	72.970, 172.970, 109.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.214 - 2.800
R-factor	0.2379
Rwork	0.234
R-free	0.27680
RMSD bond length	0.002
RMSD bond angle	0.472
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.154	1.496
Number of reflections	17421
<I/σ(I)>	15.2	1.76
Completeness [%]	99.9	100
Redundancy	15.2	13.8
CC(1/2)	0.999	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	PEG 400, MES, (NH4)2SO4, 1,4-butanediol