5GLG
The novel function of Osm1 under anaerobic condition in the ER was revealed by crystal structure of Osm1, a soluble fumarate reductase in yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97760 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.638, 109.274, 49.933 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 28.580 - 1.800 |
| R-factor | 0.18111 |
| Rwork | 0.179 |
| R-free | 0.21365 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.035 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 164160 |
| <I/σ(I)> | 33.1 |
| Completeness [%] | 94.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 30% PEG4000, 0.1M Tris, 0.2 M LiSO4 |
