5GLE

The structure of Vibrio anguillarum775 AngB-ICL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2016-01-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	140.190, 140.190, 59.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.419 - 2.100
R-factor	0.2075
Rwork	0.206
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tb4
RMSD bond length	0.007
RMSD bond angle	0.951
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	39346
<I/σ(I)>	38.1
Completeness [%]	99.9
Redundancy	21.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	2% PEG400, 0.1M Tris-base pH 8.5, 2M Li2SO4