5GLC
Crystal structure of the class A beta-lactamase PenL-tTR11 containing 20 residues insertion in omega-loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.486, 97.242, 34.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.717 - 1.601 |
R-factor | 0.1611 |
Rwork | 0.160 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gl9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.124 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.074 | 0.332 |
Number of reflections | 31195 | |
<I/σ(I)> | 15.5 | 12.263 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.2 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris pH 7.5, 30% PEGMME, 0.1M K-thiocyanate |