5GL7

Crystal structure of a truncated human cytosolic methionyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9756
Spacegroup name	P 21 21 21
Unit cell lengths	65.035, 93.030, 122.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.284 - 2.013
R-factor	0.1957
Rwork	0.194
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	zinc phazing model
RMSD bond length	0.002
RMSD bond angle	0.698
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1690)
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.013
Number of reflections	48954
<I/σ(I)>	26.1
Completeness [%]	98.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	100mM Tris pH 7.5, 200mM magnesium chloride, 5mM DTT, 21% PEG 8000