5GKX

Crystal structure of TON_0340, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2011-09-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	76.574, 80.209, 85.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.010
Rwork	0.208
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fc5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.040
High resolution limit [Å]	2.010	5.450	2.010
Rmerge	0.076	0.036	0.253
Number of reflections	32203
<I/σ(I)>	10.8
Completeness [%]	90.2	95.7	70.4
Redundancy	4.7	6.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	sodium cacodylate pH6.5. ammonium phosphate monobasic,