5GKS
Crystal structure of SLE patient-derived anti-DNA antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.356, 41.167, 155.996 |
| Unit cell angles | 90.00, 113.07, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.050 |
| R-factor | 0.21935 |
| Rwork | 0.217 |
| R-free | 0.25534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gkr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 52059 | |
| <I/σ(I)> | 22.8 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 8000, 0.1M Phosphate-citrate (pH 4.2), 0.2M NaCl |
