5GKO

Crystal structure of tripartite-type ABC transporter, MacB from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-31
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97869
Spacegroup name	P 41 21 2
Unit cell lengths	229.148, 229.148, 155.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.963 - 3.393
R-factor	0.2241
Rwork	0.223
R-free	0.25470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.866
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.393	3.400
Number of reflections	57248
<I/σ(I)>	28.2	2
Completeness [%]	99.6	100
Redundancy	8.2	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	298	1.2M Sodium Citric acid, 0.1M HEPES, 0.1M Sodium Chloride