5GKH
Structure of EndoMS-dsDNA2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.420, 92.420, 405.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.810 - 2.900 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5BSL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 22018 | |
| <I/σ(I)> | 40.4 | 28.1 |
| Completeness [%] | 92.3 | 92.9 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.16 M CALCIUM ACETATE, 80 MM SODIUM CACODYLATE, 14.4%(W/V) PEG8000, 20%(W/V) GLYCEROL |
