5GKB
Crystal Structure of Fatty Acid-Binding Protein in Brain Tissue of Drosophila melanogaster without citrate inside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-04-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97622 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 61.595, 61.595, 137.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.091 - 2.040 |
| R-factor | 0.1914 |
| Rwork | 0.186 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gge |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.100 | 0.433 |
| Number of reflections | 8811 | |
| <I/σ(I)> | 22.6 | 6.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.3 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 0.1M phosphate citrate, 1.6M NaH2PO4, 0.4M K2HPO4 |
