5GK9
Crystal structure of human HBO1 in complex with BRPF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.470, 39.321, 87.666 |
| Unit cell angles | 90.00, 122.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.26 |
| Rwork | 0.233 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2giv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.777 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.522 |
| Number of reflections | 14322 | |
| <I/σ(I)> | 18 | 3.6 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Tacsimate Tris-HCl, PEG 3350 |
