5GJX
Crystal structure of DUCK MHC I for 2.06 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.487, 63.538, 79.954 |
| Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.060 |
| R-factor | 0.21014 |
| Rwork | 0.208 |
| R-free | 0.25334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e0r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.092 | 0.568 |
| Number of reflections | 27433 | |
| <I/σ(I)> | 24.1 | 3.691 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 24 % w/v Polyethylene glycol 1500, 20 % v/v Glycerol |
