5GJ6
Functional and structural characterization of P[19] rotavirus VP8* interaction with histo-blood group antigens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.904, 129.782, 86.818 |
| Unit cell angles | 90.00, 115.90, 90.00 |
Refinement procedure
| Resolution | 33.822 - 2.388 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dwr |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.388 | 2.400 |
| Rmerge | 0.150 | |
| Number of reflections | 70312 | |
| <I/σ(I)> | 10.73 | 1.974 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 291 | 0.2M ammonium sulfate, 0.1M MES pH 6.5, 30% w/v polyethylene glycol MME 5000 |
