5GHU

Crystal structure of YadV chaperone at 1.63 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	C 2 2 21
Unit cell lengths	100.280, 127.560, 45.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.009 - 1.630
R-factor	0.1686
Rwork	0.168
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3me0 1klf 3f65
RMSD bond length	0.016
RMSD bond angle	1.590
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.838	20.842	1.720
High resolution limit [Å]	1.630	5.150	1.630
Rmerge		0.028	0.511
Rmeas	0.064	0.031	0.552
Rpim	0.017	0.008	0.154
Total number of observations	485800	17053	64105
Number of reflections	36961
<I/σ(I)>	21.6	44.2	4.4
Completeness [%]	99.5	98.5	98.7
Redundancy	13.1	13.4	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.1 M ammonium sulfate, 0.1 M sodium acetate, 0.3 M sodium formate, 30% PEG 400