5GHR

DNA replication protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97970
Spacegroup name	P 21 21 21
Unit cell lengths	48.097, 116.004, 234.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.038 - 2.509
R-factor	0.2139
Rwork	0.212
R-free	0.24880
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	45946
<I/σ(I)>	19.3
Completeness [%]	98.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG MME 5000