5GHN
Crystal structure of human MTH1(G2K/D120N mutant) in complex with 2-oxo-dATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-25 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.228, 47.271, 123.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.001 - 1.391 |
R-factor | 0.1508 |
Rwork | 0.149 |
R-free | 0.18890 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.997 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.410 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.068 | |
Number of reflections | 53100 | |
<I/σ(I)> | 49.9 | |
Completeness [%] | 96.3 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium citrate, Tris, NaCl |