5GHK

Crystal Structure Analysis of Canine serum albumin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-23
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	46.732, 118.393, 57.433
Unit cell angles	90.00, 112.78, 90.00

Refinement procedure

Resolution	34.836 - 3.200
R-factor	0.2568
Rwork	0.253
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bke
RMSD bond length	0.008
RMSD bond angle	0.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.110	0.046	0.453
Rmeas	0.120
Rpim	0.046
Total number of observations	77559
Number of reflections	11596
<I/σ(I)>	12.3
Completeness [%]	99.9	98.2	99.8
Redundancy	6.7	6.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus screen condition H1 (Molecular Dimensions)