5GHF

Transaminase with L-ala

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	78.800, 102.000, 116.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.530 - 2.000
R-factor	0.13708
Rwork	0.135
R-free	0.17426
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.869
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.530
High resolution limit [Å]	2.000
Rmerge	0.147
Number of reflections	58830
<I/σ(I)>	11.9
Completeness [%]	96.5
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	8.5	290	0.1M Tris-HCl pH 8.5, 23% PEG 3350