5GH0
Crystal structure of the complex of bovine lactoperoxidase with mercaptoimidazole at 2.3 A resolution
Replaces: 2GJ1Replaces: 5JT3Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.630, 80.667, 77.680 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1433 |
| Rwork | 0.142 |
| R-free | 0.19433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bxi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.899 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 29132 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 280 | Tris hydrochloride, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
