5GG9
Crystal structure of Mycobacterium smegmatis MutT1 in complex with 8-oxo-GTP, 8-oxo-GMP and pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.010, 83.950, 44.000 |
| Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
| Resolution | 43.940 - 1.600 |
| R-factor | 0.16732 |
| Rwork | 0.165 |
| R-free | 0.20596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fjy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.977 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 40162 | |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 0.2 M sodium acetate trihydrate, 0.1 M TRIS hydrochloride, 30% w/v PEG 4000 |
