5GG4
Crystal structure of USP7 with RNF169 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9776 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.195, 99.965, 124.299 |
| Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
| Resolution | 46.965 - 3.110 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wph |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.965 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.115 | 0.074 | 0.679 |
| Number of reflections | 35693 | ||
| <I/σ(I)> | 3.9 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 3.8 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 0.2M sodium chloride, 6% w/v PEG 8000, 0.1M Sodium cacodylate, pH 5.8 |
