5G6R
Imine reductase from Aspergillus oryzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.270, 92.440, 84.510 |
| Unit cell angles | 90.00, 100.33, 90.00 |
Refinement procedure
| Resolution | 61.820 - 1.820 |
| R-factor | 0.18703 |
| Rwork | 0.185 |
| R-free | 0.22514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a9t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.007 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.820 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.040 | 0.770 |
| Number of reflections | 53033 | |
| <I/σ(I)> | 15.9 | 1.7 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
