5G5Z
S.pneumoniae ABC-transporter substrate binding protein FusA in complex with kestose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.610, 127.820, 120.550 |
Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
Resolution | 70.145 - 2.010 |
R-factor | 0.1707 |
Rwork | 0.168 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g5y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.140 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.130 | 1.210 |
Number of reflections | 140542 | |
<I/σ(I)> | 8 | 1.4 |
Completeness [%] | 97.7 | 99.4 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 20 % PEG6000, 0.1M SODIUM ACETATE PH 5.0, 0.02 M CALCIUM CHLORIDE |