5G5X
CBS domain tandem of site-2 protease from Archaeoglobus fulgidus in complex with llama Nanobody - nucleotide-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.910, 70.910, 201.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.433 - 2.800 |
| R-factor | 0.2476 |
| Rwork | 0.246 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g5r |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.675 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.410 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 7966 | |
| <I/σ(I)> | 14.21 | 2.16 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 9.58 | 9.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M IMIDAZOLE PH 7.25, 0.2 M MGCL2, 12 % ETHANOL |
